Congratulations to Sara Ryding, Deakin University for the first chapter of her PhD thesis being published in Trends in Ecology & Evolution on “Shape-shifting: changing animal morphologies as a response to climatic warming”. Link to the paper here or here.
In this review, Sara writes about how animal appendages (ears, feet, limbs, bills, etc) are important morphological indicators of temperature and therefore potential signatures of changing climate.
Appendages have an important, but often undervalued, role in animal thermoregulation as sites of heat exchange.
This thermoregulatory role leads to geographic clines in animal morphology where animals at lower latitudes, in warmer climates, have larger appendages (a pattern known as ‘Allen’s rule’).
In this review, we discuss evidence for animals (mostly evidence in birds and mammals, although the field does extend to other animal taxa) that are shifting their morphologies to have proportionately larger appendages in response to climate change and its associated temperature increases.
It has been a real pleasure to work with Sara Ryding on this project. Full credit and thanks go to Sara for all her hard work on this paper. I helped out only a small bit, but she reviewed the field within which my lab has been conducting collaborative research since 2010. Hopefully more research will follow as she navigates the rest of the project us (Drs. Matthew Symonds and Marcel Klaassen and myself). Many late nights and early morning zoom meetings await us all. Many thanks to Deakin University and the Australian Research Council for supporting this project.
Ryding, S, Klaassen, M, Tattersall, GJ, Gardner, JL, and Symonds, MRE. 2021. Shape-shifting: changing animal morphologies as a response to climatic warming. Trends in Ecology & Evolution. DOI: https://doi.org/10.1016/j.tree.2021.07.006
Nick Sakich here. The first paper from my MSc has just been published in the Journal of Experimental Biology. The paper is entitled, “Bearded dragons (Pogona vitticeps) with reduced scalation lose water faster but do not have substantially different thermal preferences.”
In it, we examine both “wild-type” bearded dragons and two phenotypes unique to captivity (i.e. not found naturally): animals with scales of reduced prominence (known as “leatherbacks”) and completely scaleless animals (known as “silkbacks”). The following slideshow depicts the 3 variants:
There has long been speculation as to whether or not scales play a role in reducing evaporative water loss across the skin in reptiles. The seminal studies that most point to are by Licht and Bennett (1972) and Bennett and Licht (1975). Those authors looked at aberrant partially scaleless individual snakes found living in the wild and found that they did not have higher rates of water loss than “normal” snakes. However, these studies had some methodological issues, most notably sample sizes of only one (Licht and Bennett, 1972) and two (Bennett and Licht, 1975) partially scaleless snakes, respectively.
Furthermore, can reptiles (or lizards and snakes, at least) detect their rate of evaporative water loss and respond accordingly? If they can, animals with higher rates of evaporative water loss will perhaps choose cooler temperatures compared to animals with lower rates of evaporative water loss. The rate of evaporative water loss is partially thermally dependent, so for the animals this would be a way to compensate and bring their rate of evaporative water loss down.
In this study, we set out to test two hypotheses. First, we hypothesized that scales are indeed a barrier to evaporative water loss, and so leatherbacks and silkbacks would have higher rates of evaporative water loss than wild-types. Second, we hypothesized that, because of this increased rate of evaporative water loss, leatherbacks and silkbacks would have lower thermal preferences than wild-types.
We found support for our first hypothesis: both leatherbacks and silkbacks evaporated water faster than wild-types. It is likely that most of this occurs across the skin, rather than through changes in breathing or metabolism, given the simultaneous measurements we made of metabolism. This confirms what many who keep silkbacks as pets have long suspected. However, we didn’t find a statistically significant difference in thermal preference between the three phenotypes. This suggests that either leatherbacks and silkbacks can’t tell that they’re losing water faster than wild-types, or that they can tell, but they make a strategic decision to prioritize warmth over water.
I’d like to thank Arnold Liendo and Paula Rodriguez, Mandy Peck, and Kirk Riddle for supplying us with lizards for this study. I’d also like to thank Tom Eles and Wynne Reichheld, without whom keeping up with the nuts-and-bolts of animal acquisition and care would have been impossible.
Sakich, NB and Tattersall, GJ. 2021. Bearded dragons (Pogona vitticeps) with reduced scalation lose water faster but do not have substantially different thermal preferences.Journal of Experimental Biology.224 (12): jeb234427.
A link to the pdf of the manuscript can be found here (limited to 50 clicks). Otherwise, requests for pdfs can be made on Researchgate.
Licht, P. and Bennett, A. F. (1972). A scaleless snake: tests of the role of reptilian scales in water loss and heat transfer. Copeia 1972, 702-707. doi:10.2307/ 1442730
Bennett, A. F. and Licht, P. (1975). Evaporative water loss in scaleless snakes. Comp. Biochem. Physiol. A Physiol. 52, 213-215. doi:10.1016/S0300- 9629(75)80155-1
The following is a guest blog by Dr. Joshua Robertson Tabh
In my short research career, I’ve come to accept (even relish) that there are some projects that endlessly surprise; projects with shifting objectives that find you running drive-by thermal camera hand-offs along the QEW at questionable hours. The project that I’m about to describe is one of “those”. And curiously, despite the innumerable twists and turns, it just so happened to be a project with some of the most useful outcomes I’ve helped to produce. In this guest post, I’ll describe those outcomes.
But first, let’s begin in 2016. I had just begun my PhD research in avian stress physiology, and mere months before, Paul Jerem and others had released a highly intriguing protocol which suggested that the physiological stress response could be detected, and possibly quantified, in birds by simply measuring changes in body surface temperature (https://www.jove.com/t/53184/thermal-imaging-to-study-stress-non-invasively-in-unrestrained-birds). The rationale behind their protocol was that following exposure to a stressor, the sympathetic nervous system triggers vasocontriction of blood vessels at the skin (among other things), which manifests as measurable changes in skin temperature. This idea isn’t new. Rather, it likely dates back to the early 20th century or previous (e.g. Wolff and Mittelman, 1937). However, Jerem et al’s protocol was the first to show that a stress-induced change in skin temperature could be detected at the eye region in a wild bird, using infrared thermography (see Edgar et al, 2013, for a study in chickens). A clever application of thermography.
Jerem et al’s work was exciting. But a few important questions seemed to linger:
(1) how well does this stress-induced change in eye region temperature reflect circulating changes in sympathetic nervous system markers (i.e. catecholamines, like adrenaline and noradrenaline)?
So, being nagged by these questions, a team of ecophysiologists (Glenn Tattersall, Gary Burness, and Oliver Wearing), an endocrinologist (Gaby Mastromonaco), and myself sought answers.
To do so, we required an experimental approach that would allow us to measure both body surface temperature (here, at the eye region and bill) and circulating catecholamines in “stressed” and “unstressed” birds. However, measuring circulating catecholamines requires sampling blood. And since puncturing a vein with a syringe is surely sufficient to activate a physiological stress response on its own (thus rendering “unstressed” birds “stressed”) blood sampling by this standard method simply wasn’t possible. Ideally, we would fit a sample of birds with central venous catheters to permit blood sampling without capture and venipuncture. This approach could work, however, even if blood samples were to be collected effectively, catecholamines can be a pain to quantify, even for contracted labs with high-end machinery. It’s for this latter reason that we accepted the reality of leaving our first research question unanswered.
Nevertheless, we could persist with a simple experimental design to answer research questions (2) and (3); quite simply, thermographically image birds during rest (Fig 1) and during a stress exposure (for us, handling). To answer question (2), we would then quantify and compare the magnitude of stress-induced changes at the eye region and bill. And lastly, to answer question (3), we would aim to test the effect of head angle on our ability to detect stress-induced changes in eye region and bill temperature. In theory, a nice and clean approach.
Before I get to the answers of our remaining research questions, a small note on how we estimated head angle (for the interested reader).
Estimating Head Angle from 2D Image
Estimating the orientation of a 3D object from a 2D angle has been a concern for humans since photography was invented. Among mathematicians, this challenge has since acquired a formal name: the “perspective-n-point” (or “PnP”) problem. All solutions to the PnP problem first require knowledge of where, in a 2D plane, at least 3 points in an imaged object lay. We’ll call these points “landmarks”. Of course, more than 3 landmarks are best to improve estimation accuracy, but most agree that 3 will do for a reasonable guess. Next, rough dimensions of the imaged object in 3D space are needed. Such dimensions must be sufficient for one to estimate where the chosen landmarks may lie, relative to each other, in a theoretical 3D co-ordinate system known as the “world co-ordinate system”.
Once this information is collected, several geometrical approaches may be used to calculate how the imaged object must have moved or rotated such that the landmarks in 3D space overlap with those observed in 2D space (after adjusted for lens distortion). Interestingly, there is one industry with considerable investment in creating efficient geometrical approaches: virtual reality (or “VR”) gaming. Why? Because using VR gaming requires that the system can estimate the gamer’s 3D position at all times (with, interestingly, tiny infra-red lights implanted in the headset as landmarks). Thanks to this investment by the VR industry, studies developing and comparing the accuracy of geometrical solutions to the PnP problem are flourishing. It’s a perfect time for biologists like us to start taking a peak at them.
For our study, we chose to use to an approach called the “EPnP” that was first proposed by Lepetit and others in 2009 (https://link.springer.com/content/pdf/10.1007/s11263-008-0152-6.pdf). We chose this approach because it permits one to use >4 landmarks for positional estimation (thus reducing error) with little cost to computational time relative to traditional solutions. Other approaches have been lauded for improving accuracy (e.g. P3P with RANSAC) and we encourage others to pursue those approaches. For our study, however, we were interested in balancing accuracy and efficiency.
To execute the EPnP approach, we estimated the 2D position of up to 9 landmarks on a pigeon’s head by loading our thermographic images into ImageJ (Fig 2). Building a 3D model turned out to be much less time consuming – simply draw on morphometric measurements of domestic pigeons reported in literature. From these data, and EPnP algorthims, we were thus able to estimate both a 3D translation and 3D rotation of an imaged bird’s head, relative to a virtual model of a perpendicularly facing individual.
I’ll break them down by question.
Question (1): How well does this stress-induced change in eye region temperature reflect circulating changes in sympathetic nervous system markers (i.e. catecholamines, like adrenaline and noradrenaline)?
Answer: Our results suggest that, at least in our study species, surface temperature the bill is probably a better indicator of stress physiological state. I’ll explain why by referencing what we observed from data that did not control for head position. After stress exposure, bill temperature fell significantly by ~4°C after stress exposure (handling), while eye region temperature did not significantly change (Fig 3). Rather, temporal patterns in eye region temperature appeared remarkably similar between “stressed” and “unstressed” birds. Moreover, only stress-induced changes in bill temperature showed significant inter-individual variation, suggesting that if one wishes to build a metric of “stress-responsiveness” from changes in surface temperature, doing so at the bill is likely more effective than at the eye region.
Question (3): How robust and reliably detectable are stress-induced changes in body surface temperature?
Answer: It depends on where you look. After correcting for changes in head position in our birds, a significant effect of stress-exposure on eye region temperature emerged (Fig 4). This was not the case for stress-induced changes in bill temperature, which were detectable regardless of whether head position was accounted for or not. This point, we think, is particularly important for two reasons:
(1) don’t correct for changes in object position and you risk missing out on detecting biological processes, and
(2) surface temperatures of some body regions might be better indicators of your biological process of interest than others.
Take Home Message
To conclude, drawing biological inference from thermographic images is tricky. Many sources of error can get in the way of your ability to meaningfully do so, and a common one is changes in object position. As such, biologist should always remember to correct for object position when working with their surface temperature data – perhaps by using our method or another.
Tabh, Joshua KR, Burness, G, Wearing, OH, Tattersall, GJ, Mastromonaco, GF. 2021. Infra-red thermography as a technique to measure physiological stress in birds: body region and image angle matter. Physiological Reports, Accepted.https://doi.org/10.14814/phy2.14865
Dr. Joshua Robertson Tabh is a graduate of Trent University, co-supervised by Dr. Gary Burness and Dr. Gaby Mastromonaco. This research was made possible with the cooperation of the Toronto Zoo and by the watchful eye of Oliver Wearing. Since 2016, Joshua and Glenn have shared many conversations about avian physiology, imaging, and coding and Glenn invited Joshua to guest author this post after all these efforts finally reached the publication stage.
Funding for this research was provided by the Toronto Zoo Foundation, an NSERC Collaborative Research and Training Experience Program (Grant #: CREATE 481954-2016), a Howard P. Whidden grant to OHW, and an NSERC Discovery Grant to GJT (Grant # RGPIN-2014-05814).
So I usually don’t provide news about me, but this is an opportunity to thank my awesome departmental and faculty colleagues, so I’ll do so here.
I found out today I was awarded a Distinguished Scholar’s Award by my Faculty (Mathematics and Sciences). When your colleagues start emailing you congratulations, I guess you begin to take notice that something is happening. As always, when you read the nice things people say about you, it causes some self-reflection and uncertainty, but I’ll run with the peer recognition and thank my colleagues and acknowledge my own students and various collaborators who are as much part of any recognition as I would be.
Also some good news, my departmental colleague, Dr. Lori MacNeil won recognition for her outstanding teaching, both from the faculty level (Distinguished Teaching Award) and from the students (Math and Science Council Excellence in Teaching & Student Engagement Award). Congratulations Lori. It’s nice when you work with someone and see how they teach and I can attest that this is well deserved!
These are challenging times for everyone, but news like this brightens the day, and I simply want to acknowledge that whomever nominated me for this has themselves to thank as well, since I am working alongside some supportive scholars who care about our students, our research, and our involvement in public work. Clearly, I owe somebody a beer or two.
We devised a simple way to prevent gaping (i.e. temporary and rapidly reversible) and examined how strongly this influenced thermoregulatory behaviours. Interestingly, although it did significantly lower thermal selection / thermal preference behaviour the effect was quite small. We also saw some interesting changes in heat orientation behaviour. Animals that were not able to gape behaved more randomly with respect to postural orientation, whereas the control lizards tend to shy away from orienting to hot temperatures (i.e. the definition of thermoregulation is to exhibit a corrective response when moving outside the set-point range).
Alas, we don’t have any cool images to share from this study, but consider looking at some of our other papers here and here where we have examined evaporative water loss and thermal imaging in bearded dragons.
The article is part of a special issue honouring Dr. Peter Frappell, a friend and colleague in respiratory and thermoregulatory physiology. Thanks Frapps for all your input and support!
Congratulations to Ian Black for getting this published and thanks to Dr. Laura Aedy for her early work on this project.
We’re happy to share news that our paper (shareable link) was recently accepted in Methods in Ecology and Evolution! As for the nitty gritty secrets of the study, that headline was to get your attention! I’ll point out some highlights here, with some visuals to summarize the main results.
The study resulted from a visiting PhD student (Núria Montmany-Playà, @NuriaBeachy) to the lab in January 2020, immediately prior to the pandemic and lock-down. Sadly, some of the research we planned to do was not possible due to travel restrictions and embassies immediately calling back their citizens. So, this represents about the only kind of research my lab is capable of doing during a lockdown.
So, what is the paper about? Technically, it’s a methods (thus the journal choice!) and resource paper (information on empirical measurements of emissivity which I often get from colleagues) with a warning to field thermographers to “check your distance“! (Actually, Faye et al 2016 already advised this, but given various interactions I have had over the past few years, some field thermographers might not be reading all the literature – so please read Faye et al’s paper cited at the bottom of this blog).
It has become increasing common in some animal thermography studies to capture the maximum temperature from a specific region of the body (often the face or the eye) and use this as an estimate of body temperature (it is not a good estimate!) or as a proxy for vasoreactivity related to stress (which it is a better indicator of, although depends on species and body part). This effect of distance was earlier studied by Faye et al (2016), although we focus on the challenges in animal thermography in the field where animals control how close you can get! Watch the video below for the effect in action, paying attention to the maximum temperature in the eye region and how it changes as this Trumpeter swan walks closer (starting from ~15 m) and closer (~2 m) to the camera:
Here are the results plotted over time as an animated plot:
As the bird gets closer, the maximum temperature of the same animal rises from a low of ~25C to reach closer to a more realistic value of ~33C. This is an enormous range and cannot reasonably be based on vasoreactivity, when the physics of thermal optics can explain this. Read the paper for the explanation behind how spot size and distance effects interact to produce fairly large errors in thermography. Controlling for distance and/or being aware of its influences is important to any field application of thermography. I doubt too many people are trying to image animals at 10 m away given the low resolution of many thermal cameras, but as prices come down and people take their devices into the field, I am sure researchers will run into these situations.
We measured the technical error using a blackbody calibration source placed at different distances from the camera, and tested under different conditions. Below, Tb refers to the blackbody temperature (i.e. true temperature) and Ta refers to prevailing ambient temperature. Delta T refers to how the thermal camera estimates the same black body temperature (where 0 refers to the closest distance measurement). We tested this with different camera/lens combinations to give insight into how the devices function even under well controlled conditions, far different from most field applications. You can see below that the error in temperature can be as poor as 6C below actual temperature at 10 m distance, and these results are for measurements of an electronic calibration source.
We also published some simple results on how the angle of incidence influences thermography measurements (see image above of the puffin). This is also well known by thermal imaging engineers and physicists, but not likely well appreciated by biologists, so we used a Leslie cube (see image below) and adhered various bits of biological materials to a copper surface, heated the surface up and painstakingly measured the temperature, allowing us to calculate the apparent emissivity. Objects at steep angles of incidence to the camera will have a lower emissivity, which means that the apparent radiation we measure is actually a lot of background reflected radiation, and thus is a source of possible error in field thermography.
So the main message of the paper is to keep good records of how far your camera is from your object of study. Correcting for this effect is complex and beyond the scope of our study, although we report that the potential error of being 10 meters away from an object can be as high as 4 to 6C, with similar errors for measuring objects at angles greater than 50 degrees incidence.
Many thanks to Núria for her hard work on this project. Without her visit, we would not have done this. And we also want to thank the two reviewers for their hard but fair questions but also for listening to our response.
Lockdown science had me borrowing plant material from my parents, Clifford and Brenda Tattersall, so their help was crucial to the final acceptance of the manuscript!
Montmany-Playà, N. and Tattersall, GJ. 2021. Spot size, distance, and emissivity errors in field applications of infrared thermography. Methods in Ecology and Evolution.https://doi.org/10.1111/2041-210X.13563
Please consider taking part of an open repository initiative of thermal images hosted at the following website: https://trench-ir.azurewebsites.net/. If you are acquiring thermal images of plants, animals, or their environment using FLIR cameras, we would like you to share your images as part of this initiative. We welcome images from research grade cameras or from hand-held mobile phone provided the images are radiometric jpgs.
Infrared imagery offers a unique opportunity to see biophysical properties in real time. We can watch organisms heat up, cool down, and generally transfer heat back and forth throughout their environment. In the TrEnCh-IR Project, we use infrared imagery to help people see the world from a thermal perspective because we believe it’s an intuitive first step to understanding microclimate and the impacts of warming.
The TrEnCh-IR project is part of a larger initiative interested in Translating Environmental Change into organismal responses. Our goal is to build case studies of how animals are impacted by climate change to improve our approach to climate change biology education, policy, and research.
FLIR cameras are extensively used, increasingly so with the availability of FLIR thermal cameras that attach to phones. However, the cameras produce images in a non-standard format (radiometric jpgs) and analyzing the images requires purchasing expensive FLIR software. Project collaborator Tattersall has produced an open source R package (ThermImage, https://github.com/gtatters/Thermimage) that converts the images into standard formats and extracts additional data to allow analysis in commonly used and open source software such as ImageJ. Our web service makes these tools more accessible. We aim to empower more people to view the world from a thermal perspective.
Our thermal image repository will allow researchers to analyze the surface temperatures of disparate organisms in diverse environments. Education and outreach resources promote understanding how organisms experience their environment. We aim to maintain the repository long term, but can not guarantee longevity at this point. An ongoing aim is to use initial AI algorithms, potentially combined with crowd sourced landmarking, to distinguish organisms, particular body parts, and backgrounds. Our interface will allow users to explore the images to understand how organisms interact with their environments.
Currently most analyses of the impacts of climate change on organisms are based on air temperatures, but body temperatures of ectotherms can differ from air temperatures by tens of degrees. Additionally, the characteristics and behaviours of organisms can result in their experiencing different body temperatures even in the same environment with repercussions for species interactions. Moving beyond air temperatures to consider body and surface temperatures may thus be essential to accurately forecasting climate change impacts. Thermal images provide compelling visual examples of why we need to move beyond air temperatures in examining climate change impacts as well as data that can inform approaches for modelling how organisms interact with their environment.
Dr. Lauren Buckley, University of Washington, Professor
Abigail Meyer, Lead Developer & University of Washington Research Scientist
Dr. Glenn Tattersall, Brock University Professor & Thermal Biologist
Site development based with University of Washington, in the Department of Biology.
This speaks to ~8 years of winter efforts to test this technique out in the lab and in the field! Hopefully we will publish soon. I’m not used to the CBC scooping us, but they don’t have to write the manuscripts and do the stats, so maybe I can excuse them.
These efforts are all related to a head starting project initiated by Anne Yagi on the Massasauga, taking physiological and behavioural data performed in careful lab experiments, testing these for 1-2 winters, then expanding to larger sample sizes in subsequent years to lead to the 9 minute videos above.
Many thanks to 8Trees Inc, Anne Yagi, Dr. Katharine Yagi and all of the animal care and field assistants that make Anne’s work possible (Theresa Bukovics, Tom Eles, Shawn Bukovac, Matt Jung, and many others).
At long last, resulting from herculean efforts of a number of former students, our paper is published. Out today in Royal Society Open Science, our paper entitled: “Hydrogen sulfide exposure reduces thermal set point in zebrafish” represents the efforts of two honours students (JC Shaw and CD Dobell) and the writing and analytical skills of a great PDF and colleague (DA Skandalis).
Here is a link to the study and full citation:
Skandalis DA, Dobell CD, Shaw JC, Tattersall GJ. 2020 Hydrogen sulfide exposure reduces thermal set point in zebrafish. R. Soc. Open Sci. 7: 200416.
We tested whether dissolved H2S in the water will alter thermal preference. Previously, work in mice has suggested that mice could be induced to adopt a “hibernation-like” state, although there was some doubt (in the literature) as to whether H2S signalled a change in thermoregulatory state or simply acted as a metabolic inhibitor. By testing this in zebrafish, we could test formally whether they prefer cooler temperatures with H2S exposure, and they did. Not only did they choose to cool down, but they continued to make thermoregulatory decisions, swimming back and forth between cool and warmer water, suggesting they are still making thermoregulatory decisions and not simply caught in the cold water. So…yeah, complicated. H2S might induce a behavioural anapyrexia (a lowered thermal set-point). We discuss the potential environmental and neurophysiological context in the paper for those interested. The rotten egg reference is to the smell of H2S gas.
To conduct this study, we used a system built by Brock University’s Technical Services and employed in our research lab that allows us to track fish in a two chamber thermal shuttle box:
This setup allows us to heat and cool a tank and track the fish’s choices over time. Here is a thermal image depicting an earlier version of the shuttle box (correcting the spill over of warm-water in the centre can be corrected using baffles and a circular chamber system, but I haven’t taken a new picture with the thermal camera during the pandemic lockdown):
There was some considerable interest in developing H2S as a therapeutic to put mammals and/or tissues/organs into a suspended state. It is intriguing that animals like zebrafish that can behaviourally regulate body temperature continue to do so under this exposure. Anaprexic stategies are commonly seen in ectotherms and perhaps by hijacking an innate signalling system, H2S evokes this response.
Congratulations, Nick! We just heard from the Faculty of Graduate Studies that Nick has received the 2020 Fall Distinguished Graduate Student Award for his MSc project. A well deserved award and a credit to Nick’s hard work.